mtor pathway  (MedChemExpress)

Journal: Journal of Cellular and Molecular Medicine

Article Title: YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR – HIF1α – LHDA Axis

doi: 10.1111/jcmm.71105

Figure Lengend Snippet: YTHDF3 regulates the glycolysis level of breast cancer through the mTOR–HIF1α–LDHA axis. (A) Relative mRNA expression of HIF1α in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (B) the correlation between YTHDF3 and HIF1α was predicted using the GEPIA website; (C) expression of key proteins in the mTOR–HIF1α–LDHA axis in shNC and shYTHDF3 groups in MDA‐MB‐231 and MCF‐7 cells; (D) after adding activator MHY1445, the growth ability of cancer cells in shNC and shYTHDF3 groups was detected by CCK‐8 assay; (E) the lactate level of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485; and (F) expression of proteins of cancer cells in shNC and shYTHDF3 groups treated with activator MHY1485.

Article Snippet: The activator of the mTOR pathway, MHY1485, was obtained from MedChemExpress (Shanghai, China) at a concentration of 10 mM.

Techniques: Expressing, CCK-8 Assay

Journal: Frontiers in Oncology

Article Title: Glutamine alleviates radiation-induced intestinal injury in rats via the mTOR/Notch1 axis

doi: 10.3389/fonc.2026.1735401

Figure Lengend Snippet: Effects of Gln and RAPA on the mTOR/Notch1 axis and MUC2 expression in HT-29 cells. (A) The expression of mTOR, p-mTOR, Notch1, and GAPDH. (B) Immunofluorescence analysis of MUC2 in HT-29 cells (800×). Data are presented as mean ± SD (n = 3). One-way ANOVA was carried out followed by Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: To investigate the role of mTOR/notch1 pathway on Gln radio-resistance effects in colon, HT-29 cells were treated with the 10 μmol/L Jagged-1 (MCE, USA) , which activates the Notch1 receptor.

Techniques: Expressing, Immunofluorescence

Journal: Frontiers in Oncology

Article Title: Glutamine alleviates radiation-induced intestinal injury in rats via the mTOR/Notch1 axis

doi: 10.3389/fonc.2026.1735401

Figure Lengend Snippet: Effects of Gln and Jagged-1 on the mTOR/Notch1 axis and MUC2 expression in HT-29 cells. (A) The expression of mTOR, p-mTOR, Notch1, and GAPDH. (B) Immunofluorescence analysis of MUC2 in HT-29 cells (800×). Data are presented as mean ± SD (n = 3). One-way ANOVA was carried out followed by Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: To investigate the role of mTOR/notch1 pathway on Gln radio-resistance effects in colon, HT-29 cells were treated with the 10 μmol/L Jagged-1 (MCE, USA) , which activates the Notch1 receptor.

Techniques: Expressing, Immunofluorescence

Journal: Biology

Article Title: Primary Follicle Paces Fish Ovarian Maturation Developmental Progression via the Enhancement of Notch and mTOR

doi: 10.3390/biology14121752

Figure Lengend Snippet: Regulatory effect of Notch/mTOR on the developmental process of PFs in zebrafish. ( A ) qRT-PCR to detect the expression of notch2 , akt2 , and mtor genes in different types of PFs in zebrafish. ( B ) Immunohistochemistry (IHC) to detect the Notch and mTOR proteins localization in the ovary and expression of different types of PFs of zebrafish; scale bar, 50 μM; ( C , F , I ) Histological observation of the ovary in the treated group with IMR-1, P529, and dietary amino acid supplementation; scale bar, 100 μM. ( D ) The hey1 , hey2 , hes , mtor , and s6k1 mRNA levels in the ovary tissues of IMR-1-treated groups. ( E ) The Notch, mTOR, and p-mTOR protein levels were expressed in the ovarian tissues of IMR-1-treated groups. ( G ) The s6k1 and notch mRNA levels were expressed in the ovarian tissues of the P529-treated groups. ( H ) The mTOR, p-mTOR, S6K1, and p-S6K1 protein levels of expression in the ovarian tissues of the P529-treated groups. ( J ) The mtor , s6k1 , and notch mRNA levels of expression in the ovarian tissues of the amino acid addition group. ( K ) The mTOR, p-mTOR, S6K1, P-S6K1, and Notch protein levels were expressed in the ovarian tissues of the amino acid addition group. “*”: p < 0.05; “**”: p < 0.01; “***”: p < 0.001 ; “****”: p < 0.0001. (means ± SD of relative expression; n = 3 for each group).

Article Snippet: The Notch signaling pathway inhibitor Inhibitor of Mastermind Recruitment-1 (IMR-1) [ , ] (Selleck, S8280, Selleck Chemicals, Houston, TX, USA) and the mTOR signaling pathway inhibitor Palomid 529 (P529) [ , ] (Selleck, S2238) were separately dissolved in DMSO to prepare their 10 mM stock solutions.

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemistry